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Pep-Overlap
In Pep-Overlap arrays, peptides are derived from and walk downstreams along the aa sequence of a target protein and two consecutive/neighboring peptides have several aa overlap (1~3 aa shift/offset is usually recommended for fine-mapping), and these peptides are also called overlapping peptides.
By incubating Pep-Overlap array with bait proteins (such as serum antibodies, pure and partial purified proteins, and other biomolecules), the (sub-)domains (such as epitopes of an antigen, binding motifs for a bait protein, etc) in the target protein can be quickly identified.
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Specifications:
- Fmoc Solid-Phase Spot Synthesis on PEG-Grafted Cellulose Membrane
- 10~50nmol per spot, >85% purity
- Strong Binding of Peptides on Membrane
- Low Background: Superior Image Quality
- Straightforward workflow (flowchart and chemicals).
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Applications:
- Epitope Mapping: Antigens are laid in the form of overlapping peptides on peptide array which can be incubated with sera, and then detected by secondary antibody. Useful for antibody profiling, sub-unit vaccine development.
- immune monitoring of vaccine responses
- Mapping of binding motifs for protein-protein interaction (substrate-enzyme, ligand-receptor, etc): Target protein is laid on peptide array, incubated with bait protein and then detected by the antibody against bait protein; vice versa for finding binding motifs on bait proteins.
- Binding motifs of a proteins for binding with other biomolecules: Protein-nucleic acid, protein-extracellular matric etc. Other biomolecules must be detected by either direct labeling (e.g. biotin, fluorochrome, isotopes etc) or indirect detection (e.g. antibodies, etc).
- Screening for serum biomarkers
Accomplishments
- a peptide interfering with polymerization of Tau protein
- a peptide designed for C5a inactivation
- Haparin binding sites of hepatic lipase
- more...
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Pricing:
- $1/amino acid residue (USD for non-Canadian customers)
- Calculation of number of amino acid residues: for example, to make a 12-mer peptide array of a protein of 400 amino acid residues at 2 AA frameshift (10 overlapping AAs), the number of total peptides is (400-12)/2=194, then the number of total amino acid residues is 194×12=2328.
(Click here to make a quick request for quotes) |
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