• General
• Pep-Overlap
• Pep-Scan
• Pep-Optima
• Pep-Hit
• Pep-Phos
• Pep-Lyse
• Pep-Well

Pep-Phos

Pep-Phos can be divided into Pep-Kinase and Pep-Phosphotase

Pep-Kinase: Protein kinase is a type of enzyme that transfers phosphate groups from high-energy donor molecules, such as ATP, to specific proteins. Protein kinases are used extensively to transmit signals and control complex processes in cells. Up to 518 different kinases have been identified in humans. The enormous diversity, as well as their role in signaling, makes them an object of study.

Carefully selected from our 11,000 phosphorylated site library, we offer 3 specific substrate arrays for  threonine, serine and tyrosine kinases to help scientists characterize substrate specificity of kinases, or identify autophosphorylation sites, or screen inhibitors, or compare kinases. We also custom make kinase substrate arrays to suit researcher's requirements.

Pep-Phosphotase: Dephosphorylation also plays an important role in signal transduction and consequent biological functions. Contrary to Pep-Kinase, Pep-Phosphotase has phosphopeptides (P-tyrosine, or P-threonine, or P-serine) synthesized on arrays. After incubation with phosphotase, the signal reduction in detection by phosphopeptide-specific dye or antibody indicates dephosphorylation events.

Figure of Pep-Kinase

Specifications

• Over 11,000 pre-identified phosphorylated sites in over 3200 substrates from human, mouse and rat
• Specific substrate arrays for threonine, serine and tyrosine kinases, or specific phosphopeptide arrays for phosphotases
• Combinatorial library of substrates are available to identify substrates for a given kinase or phosphotase
• 10~20nmol for each substrate on array, sufficient for high-throughput screening
• Excellent consistency, reproducibility and reliability

Pre-identified Phosphorylation Library:

Applications:

• Identification of kinases or phosphotases, using pre-defined substrates
• Substrate profiling of a given kinase or phosphotase, using pre-identified substrates
• Fingerprinting difference in the substrate specificities among different kinases or phosphotases
• Evaluation of inhibitors of kinases or phosphotases
• Identification of substrate sequence, or optimization of substrates, or characterization of the target
  phosphorylation consensus motifs of a kinase or phosphotase, using a subsitutional or combinatorial library of substrates (which vary the amino acid residues at each position flanking a phospho-acceptor residue)
• Mapping phosphorylated sites of a protein (a protein substrate of a certain kinase is known, and the aim is to identify the actual site of phosphorylation within the target protein), using overlapping peptides spanning whole AA sequence which are then incubated with kinases and detected by anti-phosphopeptide antibodies or dyes.
• Elucidation of signal transduction pathways
• more...