In the post-genomic era, scientists have shifted their research focus from genomic studies onto proteomics. The study of the interaction of protein with other biomacromolecules such as proteins, nucleic acids, and polysaccharides is of great interest, and peptide array is one of key and top-notch technologies for proteomic research.
The FMOC Solid-Phase SPOT synthesis is well known as a rapid and robust method to generate peptide libraries on membrane supports – particularly on cellulose sheets, which deliver speed, throughput and consistency in proteomics.
Harnessing automatic robots, PepMetric can synthesize hundreds and thousands of custom designed peptides in situ on specially modified cellulose membrane (Figure 1). As a result, peptide array will help scientists high-throughput proteomic research.
Peptide Array is widely used in drug screening, target validation, epitope mapping, vaccine development, and structure-activity studies. There is an ever increasing demand from large pharmaceutical companies due to the expanding interest in peptide drugs.
In the past 20 years, PepMetric has accumulated rich experience in peptide array and its downstream applications, and successfully designed/discovered a variety of peptides in fields of immunology, virology, proteomics, clinical diagnostics, pharmaceutics and therapeutics.
Illustration of Peptides on Array
Features:
Highly customizable to suit customers' demands. Our expert can also help customers design arrays
High peptide integrity and fidelity (>70% purity for up to 14~16 amino acids, as confirmed by HPLC)
Combinatorial parallel synthesis for constructing large arrays of peptides, faster to make and more cost effective than whole protein arrays
10-100 nmole scale per spot with high purity, batch-to-batch reproducibility
Spatially addressable peptides to mimic a biological environment
Versatility of addition of many building blocks such as D-aa, phosphorylated aa, biotin-aa, and other non-natural aa
Various linkers providing sufficient spacing to conserve the accessibility and activity of N-terminal residues
Huge epitope, kinase, protease substrate database
Unique peptide design algorithm with high success rate
Multiple detection systems applicable to peptide array
No special lab equipment requirements for downstream experiments, can be performed at regular biological laboratories
Pep-Overlap: Overlapping peptides from any protein sequence for a quick mapping of interacting motifs of protein with other biomolecules (receptor-ligand, protein-protein, protein-nucleic acid, etc), antigenetic clusters/epitopes (antigen-antibody).
Pep-Scan: substitution of any aa in peptides to detect critical aa, more efficient and faster than traditional site-directed mutagenesis.
Pep-Optima: fast generation of combinatorial library of any aa residues in a peptide for optimization of their best combination, useful for peptide drug discovery, substrate optimization/discovery, etc
Pep-Hit: based on target protein sequence, this unique algorithm for peptide design generates hundreds of peptide candidates for drug discovery
Pep-Phos: peptide array of kinase substrates (over 11,000 substrate library for selection) for kinase or phosphotase profiling
Pep-Lyse: protease substrates on membrane for evaluation of substrate specificity and identification of protease class and substrates.
PEP-Well: peptides in 96-well microplate for high throughput screening
Pep-Overlap
Good for protein-protein interation research ... (more)
Pep-Hit
Unique algorithm for peptide drug discovery ... (more)
Pep-Optima
Fast optimization of AA sequence for any given peptides .... (more)
